Evidence for reduced reproductive success (RS) of wild spawning hatchery-reared fish invites serious consideration with regard to the detrimental effects on subsequent generations of wild populations. Mate choice was evaluated as a potential mechanism contributing to these observed RS differences using a previous pedigree of wild spawning hatchery-reared and wild origin coho salmon (Oncorhynchus kisutch). Genetic variance at immune-relevant genes was used as a metric to examine mate choice. Two years (2005 and 2006) of three wild spawning mate pair classes were examined: wild x wild (W x W), hatchery x hatchery (H x H), and wild x hatchery (W x H). We tested for: (1) a departure from random expectations with regard to mate pair allelic diversity at immune-relevant markers, (2) a correlation between immune-relevant gene diversity and mate pair RS, and (3) distinguishable differences between mate choice strategies used by hatchery-reared and wild origin coho. Eight immune-relevant gene-linked microsatellite markers were used to evaluate mate choice; four linked to immune-relevant expressed sequence tags (ESTs) and four linked to the Major Histocompatibility Complex (MHC). We found evidence for non-random mating between 2006 W x H mate pairs at BHMS429, an MHC-linked marker, and at SsalR016TKU, an immune-relevant EST-linked marker, which was identified as a vasodilator-stimulated phosphoprotein. Non-random mating was also evident between 2005 H x H pairs at SsalR015TKU, an immune-relevant EST-linked marker, though no putative gene was identified. All other pair classes did not display a significant mate choice signature. We found a significant correlation between mate pair RS and immune gene diversity among 2005 and 2006 W x W mate pairs as well as 2006 W x H mate pairs. Notably, H x H mate pair RS was not correlated to immune gene diversity in either year. Results suggest that mate choice and genetic compatibility may influence fitness of wild spawning coho.

Authors: 
Amelia C. Whitcomb
Short Description: 
Evidence for reduced reproductive success (RS) of wild spawning hatchery-reared fish invites serious consideration with regard to the detrimental effects on subsequent generations of wild populations. Mate choice was evaluated as a potential mechanism con
Product Number: 
ORESU-Y-12-005
Entry Date: 
Wednesday, June 19, 2013
Price: 
NA
Length: 
107 pp.
Size and Format: 
8 1/2 x 11, online
Department/University: 
Fisheries Science, Oregon State University
Degree: 
Master of Science
Year of Publication: 
2012
How to Order: 

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