Development of a PCR assay to detect cyprinid herpesvirus 1 in koi and common carp

Cyprinid herpesvirus 1 (CyHV1) infects all scaled and color varieties of common carp Cyprinus carpio, including koi. While it is most often associated with unsightly growths known as ‘carp pox,’ the underlying lesion (epidermal hyperplasia) can arise from a variety of disease processes. CyHV1-induced epidermal hyperplasia may occur transiently in response to water temperature, and thus histopathology cannot be used in isolation to assess CyHV1 infection status.

Detection of ORF6 protein associated with latent KHV infection

Koi herpesvirus (KHV) is highly pathogenic to Cyprinus carpio. KHV can also become latent in recovered fish and reactivate from latency under stressful conditions. Understanding KHV latency is important for development of strategies against herpesvirus latent infection. Our previous studies found KHV ORF6 mRNA is detectable during latent infection. In this study, ORF6 protein expression was investigated by a polyclonal antibody specific to ORF6 peptide.

Persistent spatial structuring of coastal ocean acidification in the California Current System.

The near-term progression of ocean acidification (OA) is projected to bring about sharp changes in the chemistry of coastal upwelling ecosystems. The distribution of OA exposure across these early-impact systems, however, is highly uncertain and limits our understanding of whether and how spatial management actions can be deployed to ameliorate future impacts.

A self-cleaning biological filter: How appendicularians mechanically control particle adhesion and removal

Appendicularians are ubiquitous marine grazers that use tangential filtration to collect micron and submicron prey. The food-concentrating filter (FCF) is the primary determinant of appendicularian prey selectivity, but the precise means by which it concentrates and conveys particles to the pharyngeal filter remain poorly understood.

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